Journal: ACR Open Rheumatology

Article Title: The Follistatin‐like Protein 1 Pathway Is Important for Maintaining Healthy Articular Cartilage

doi: 10.1002/acr2.11155

Figure Lengend Snippet: Primer sequences

Article Snippet: Goat polyclonal antibodies were used to detect human FSTL1 and MMP13 (R&D Systems).

Techniques: Sequencing

Journal: ACR Open Rheumatology

Article Title: The Follistatin‐like Protein 1 Pathway Is Important for Maintaining Healthy Articular Cartilage

doi: 10.1002/acr2.11155

Figure Lengend Snippet: Follistatin‐like protein 1 (FSTL1) expression regulates chondrocyte phenotype. CH‐hTERT/E6/E7 chondrocytes were transduced with lentivirus encoding FSTL1 short hairpin RNA shRNA) or scrambled shRNA (control). Chondrocyte differentiation was performed in the presence of transforming growth factor β. A , After 21 days of culture, FSTL1, COL2A1, ACAN, MMP13, COL10A1, and RUNX2 were assayed by real‐time PCR. The mean + SEM is shown (n = 3; FSTL1 * P = 0.01, COL2A1 * P = 0.01, ACAN ** P = 0.028, MMP13 * P = 0.01, COL10A1 *** P = 0.0015, RUNX2 **** P = 0.0039; two‐tailed t test). B , After 7, 14, or 21 days of culture, cell lysates were assayed by Western blotting for FSTL1, MMP13, or actin.

Article Snippet: Goat polyclonal antibodies were used to detect human FSTL1 and MMP13 (R&D Systems).

Techniques: Expressing, Transduction, shRNA, Control, Real-time Polymerase Chain Reaction, Two Tailed Test, Western Blot

Journal: ACR Open Rheumatology

Article Title: The Follistatin‐like Protein 1 Pathway Is Important for Maintaining Healthy Articular Cartilage

doi: 10.1002/acr2.11155

Figure Lengend Snippet: Overexpression of Follistatin‐like protein 1 (FSTL1) enhances COL2A1 gene expression. CH‐hTERT/E6/E7 chondrocytes were infected with adenovirus encoding FSTL1 (FSTL1) or a control virus lacking a transgene (control). Chondrogenic differentiation was performed in a 21‐day pellet culture with transforming growth factor β (TGFβ). FSTL1 ( A ), COL2A1 ( B ), and MMP13 ( C ) expression was assessed by real‐time PCR. The mean + SEM is shown (n = 3; FSTL1 * P = 0.014, COL2A1 ** P = 0.0098, MMP13 P = 0.55; two‐tailed t test).

Article Snippet: Goat polyclonal antibodies were used to detect human FSTL1 and MMP13 (R&D Systems).

Techniques: Over Expression, Gene Expression, Infection, Control, Virus, Expressing, Real-time Polymerase Chain Reaction, Two Tailed Test